Application Notes

Our Application Notes below represent just some of the work we have completed using the Axcend Focus LC®. Please complete the request form below if you have an application of interest that you do not see listed.

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Application Notes

TRACE LEVELS OF BTEX IN WATER USING HAND-PORTABLE HPLC

This brief application note demonstrates the use of hand-portable capillary HPLC by the Axcend Focus LC® for BTEX compounds in water––specifically, with a 5 μL external injection loop and UV-absorption detection at 255 nm.

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INTACT PROTEIN ANALYSIS BY RPLC USING HAND-PORTABLE HPLC

This brief application note demonstrates the use of hand-portable capillary HPLC by the Axcend Focus LC® for intact protein analysis. Specifically, the separation of five test proteins in the range of 5.8 to 45 kDa is shown using reversed phase (RP) conditions. Other protein samples can be analyzed using the Axcend Focus LC® merely by adapting established conventional high performance LC methods to the capillary column format. For the complete Application Note, click on the READ MORE button.

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12-HOUR DISSOLUTION OF EXTRA STRENGTH EXCEDRIN® BRAND ANALGESIC IN 200mL OF MEDIA WITH THE AXCEND LC®

The Axcend Focus LC® High-Performance Liquid Chromatography system (HPLC) delivers repeatable analyses of Active Pharmaceutical Ingredient (API) dissolution. In this test, an Extra Strength Excedrin® analgesic tablet was dissolved, without stirring, in a beaker containing 200 mL of media [97% water; 3% acetonitrile (ACN); and <0.1% trifluroacetic acid (TFA)].

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DETERMINATION OF 5 HEMP-BASED CANNABINOIDS & 12 STANDARD CANNABINOIDS BY HAND-PORTABLE HPLC

This application note demonstrates the ability of the Axcend Focus LC® portable HPLC to detect and separate 5 principal cannabinoids present in 50 mg of a hemp-variant of cannabis flowers (dry weight) and 12 additional cannbinoids present in a Restek standard. Run-times for both analyses of ~18 minutes with a 50:50 mixture of ACN 0.3 M NH4Ac buffer pH 4.6 (Mobile Phase A) and MeOH 50% ACN (Mobile Phase B).

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TWO METHODS FOR QUICK ANALYSIS OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) USING AXCEND FOCUS LC®

The Axcend Focus LC® was used to separate polycyclic aromatic hydrocarbons (PAHs) in ~12 and ~15 minutes, respectively, with separate methods on short (10 cm x 150 µm i.d.) capillary columns packed with 1.7 µm monomeric and 3.0 diameter polymeric C18 particles.

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FIVE PRINCIPAL CANNABINOIDS SEPARATED AND DETECTED IN NINE MINUTES USING THE AXCEND FOCUS LC®

Five cannabinoids were separated and detected in ~nine minutes using the Axcend Focus LC® High-Performance Liquid Chromatography system (HPLC): Cannabidiolic Acid, Cannabidiol, Cannabinol, Delta(9)-Tetrahydrocannabinol, and Delta(9)-Tetrahydrocannabinolic Acid.

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Have an Application of interest that is not addressed in our Application Notes library? Please complete this form so we can learn more about your Application and investigate how we can work with you to get the results you need.

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Provo, UT USA 84604

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