A simple analysis of caffeine and benzoic acid in soft drinks using the compact Axcend Focus LC® is demonstrated. Linear regression of the calibration data gave R2 values of 0.9993 and 0.9998 for caffeine and benzoic acid, respectively. Analysis of a commercial soft drink gave 228 ppm caffeine (RSD 1.1%) and 184 ppm benzoic acid (RSD 1.8%). Solvent usage was estimated to be approximately 28 μL per analysis.

This brief application note demonstrates the use of hand-portable capillary HPLC by the Axcend Focus LC® for BTEX compounds in water––specifically, with a 5 μL external injection loop and UV-absorption detection at 255 nm.

This brief application note demonstrates the use of hand-portable capillary HPLC by the Axcend Focus LC® for intact protein analysis. Specifically, the separation of five test proteins in the range of 5.8 to 45 kDa is shown using reversed phase (RP) conditions. Other protein samples can be analyzed using the Axcend Focus LC® merely by adapting established conventional high performance LC methods to the capillary column format. For the complete Application Note, click on the READ MORE button.

The Axcend Focus LC® High-Performance Liquid Chromatography system (HPLC) delivers repeatable analyses of Active Pharmaceutical Ingredient (API) dissolution. In this test, an Extra Strength Excedrin® analgesic tablet was dissolved, without stirring, in a beaker containing 200 mL of media [97% water; 3% acetonitrile (ACN); and <0.1% trifluroacetic acid (TFA)].

This application note demonstrates the ability of the Axcend Focus LC® portable HPLC to detect and separate 5 principal cannabinoids present in 50 mg of a hemp-variant of cannabis flowers (dry weight) and 12 additional cannbinoids present in a Restek standard. Run-times for both analyses of ~18 minutes with a 50:50 mixture of ACN 0.3 M NH4Ac buffer pH 4.6 (Mobile Phase A) and MeOH 50% ACN (Mobile Phase B).

The Axcend Focus LC® was used to separate polycyclic aromatic hydrocarbons (PAHs) in ~12 and ~15 minutes, respectively, with separate methods on short (10 cm x 150 µm i.d.) capillary columns packed with 1.7 µm monomeric and 3.0 diameter polymeric C18 particles.

Five cannabinoids were separated and detected in ~nine minutes using the Axcend Focus LC® High-Performance Liquid Chromatography system (HPLC): Cannabidiolic Acid, Cannabidiol, Cannabinol, Delta(9)-Tetrahydrocannabinol, and Delta(9)-Tetrahydrocannabinolic Acid.