PharmSci360

Join us at AAPS PharmSci360! AAPS is the convener of the pharmaceutical science community, bringing together thousands of scientists from across the world and the drug development process. For them, PharmSci 360 combines all the energy of a large scientific conference with the intimacy of a small niche meeting because of its unique programming structure.

Event Details

DATES: November 9-12, 2025

LOCATION: San Antonio, Texas

BOOTH: 3024

Our Presentations

Be sure to attend our presentations throughout the conference to hear the latest data and insights from our experts and collaborators.

Adapting USP Monographs to the Capillary Scale using USP 621

POSTER | November 12, 2025 | 11:30 AM - 12:30 PM | W1130-02-11 | Dr. Garrett Hellinghausen, Axcend

The United States Pharmacopeia (USP) offers numerous pre-validated monographs of pharmaceutically relevant compounds which are used in regulated environments around the world. With environmental concerns impacting major pharmaceutical manufacturers, labs are working to reduce their consumption of harmful mobile phases in LC analysis. One method of solvent reduction is the use of capillary-scale liquid chromatography, where columns with an inner diameter (i.d.) between 0.075 and 0.5 mm are operated between 0.1 and 10 µL/min. The considerable cost and time required for method revalidation has previously limited the adoption of capillary-scale LC in regulated environments. By using guidelines established in USP < 621 > [1], monographs can be adapted to the capillary scale without the need for full revalidation. This work demonstrates the adaptation of a monograph for hydrochlorothiazide [2] on both analytical and capillary scale. Both scales were able to meet the system suitability criteria of the monograph. The capillary-scale system was able to reduce the total solvent consumption by 99.6% when compared to the initial monograph. This work marks a significant step in the integration of capillary-scale LC into regulated environments.