Eastern Analytical Symposium

Find us at this year's Eastern Analytical Symposium (EAS)! EAS has been the leading conference in the analytical and pharmaceutical sciences for over 60 years, showcasing the full spectrum of analytical chemistry and related disciplines from art conservation to pharmaceutical development because inspiration for laboratory solutions can come from anyone, anywhere. 

Event Details

DATES: November 17-19, 2025

LOCATION: Plainsboro, New Jersey

BOOTH: M5

Our Presentations

Be sure to attend our presentations throughout the conference to hear the latest data and insights from our experts and collaborators.

Greener Routine HPLC Analysis: Translation of USP Monographs to the Capillary Scale

PRESENTATION | November 18, 2025 | 9:40 AM - 10:00 AM | Dr. Samuel Foster, Axcend

High performance liquid chromatography (HPLC) is widely used for routine analysis in many industries. Analytical scale systems are commonly employed using columns with an inner diameter (i.d.) between 4.6 mm and 2.1 mm. Each of these systems can consume ~50 L of solvent per year. Often these solvents contain toxins including trifluoracetic acid (TFA), methanol, and acetonitrile. By translating existing methods to the capillary scale using columns of 0.1 mm to 0.3 mm i.d., separations can be performed that consume only ~100 mL of solvent per year per instrument. This reduction in solvent consumption can result in significantly reduced environmental impact as well as reduction in operating expenses. In this work, a USP monograph was translated to the capillary scale using USP 621 guidelines. Capillary separations were compared to analytical scale separations as described in the monograph, demonstrating comparable separations. Additional general guidance is presented for method translation of analytical scale HPLC separations to capillary scale separations including column selection, injection volume, and gradient programming. Capillary scale HPLC is essential in the pursuit of greener separations without the need for changes in mobile phase composition or additives.