Understanding Capillary LC - Part 4: Reducing Extra Column Effects

The fluidic pathway plays a critical role in achieving high-resolution separations at the capillary scale. In this fourth installment of Understanding Capillary LC, Dr. Sam Foster explores how extra-column band broadening impacts chromatographic performance and why its effects are amplified in low-flow systems.

Attendees will learn practical strategies for minimizing these effects—such as using narrow internal diameter tubing, face-sealing fittings, and zero-dead-volume unions—and see how the Axcend Focus LC® incorporates these principles in its design. Whether you’re troubleshooting peak distortion or optimizing a new capillary method, this session will help you better understand how fluidic design influences your results.

• Understand what causes extra-column band broadening and its impact
• Learn how low-flow conditions magnify these effects at the capillary scale
• Explore design strategies to reduce dispersion and maintain efficiency
• See how the Focus LC’s fluidic pathway minimizes dead volume and maximizes resolution

Webinar

Speaker

Samuel Foster, Ph.D.
Application Scientist
Axcend

Samuel Foster completed his Ph.D. in Pharmaceutical Chemistry from Rowan University in 2025. His research has focused on the development and application of capillary scale liquid chromatography instrumentation. He currently works at Axcend as an application scientist focusing on the development of chromatographic workflows for a variety of analyte classes including oligonucleotides, monoclonal antibodies, and drugs of abuse.